1EY2

HUMAN HOMOGENTISATE DIOXYGENASE WITH FE(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-25
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 63 2 2
Unit cell lengths	157.809, 157.809, 95.147
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.193
Rwork	0.193
R-free	0.24200
RMSD bond length	0.010
RMSD bond angle	1.930
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.083	0.412
Total number of observations	187375 *
Number of reflections	31495
<I/σ(I)>	19.3
Completeness [%]	98.7	89.9
Redundancy	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *		298	Ammonium Sulfate, Imidazole, FeSO4, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	1.5-2.0 (M)