1EER
CRYSTAL STRUCTURE OF HUMAN ERYTHROPOIETIN COMPLEXED TO ITS RECEPTOR AT 1.9 ANGSTROMS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.400, 79.300, 136.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.31800 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 27.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.044 * | 0.377 * |
Total number of observations | 251681 * | |
Number of reflections | 49480 | |
<I/σ(I)> | 19.1 | 2.5 |
Completeness [%] | 97.0 | 82.9 |
Redundancy | 5.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG1500 | 32 (%) | |
2 | 1 | reservoir | ammonium sulfate | 0.28 (M) | |
3 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |