1E7U
Structure determinants of phosphoinositide 3-kinase inhibition by wortmannin, LY294002, quercetin, myricetin and staurosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.327, 67.664, 106.650 |
Unit cell angles | 90.00, 95.05, 90.00 |
Refinement procedure
Resolution | 72.380 - 2.000 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1QMM |
RMSD bond length | 0.007 |
RMSD bond angle | 21.700 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.380 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 94.200 * |
Total number of observations | 227525 * | |
Number of reflections | 68705 | |
<I/σ(I)> | 16.01 | 1.6 |
Completeness [%] | 97.4 | 94.2 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 * | 17 * | used hair seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 0.1 (M) | |
2 | 1 | reservoir | ammonium sulfate | 250 (mM) | |
3 | 1 | reservoir | PEG4000 | 19 (%) |