1D8T

CRYSTAL STRUCTURE OF ELONGATION FACTOR, TU (EF-TU-MGGDP) COMPLEXED WITH GE2270A, A THIAZOLYL PEPTIDE ANTIBIOTIC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1998-06-18
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	133.470, 45.170, 144.000
Unit cell angles	90.00, 94.64, 90.00

Refinement procedure

Resolution	24.440 - 2.350
R-factor	0.2
Rwork	0.200
R-free	0.24600
RMSD bond length	0.007
RMSD bond angle	25.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA)
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.440	2.410
High resolution limit [Å]	2.350	2.350
Rmerge	0.032	0.108
Total number of observations	55193 *
Number of reflections	31214
<I/σ(I)>	16.4
Completeness [%]	86.3	60.7
Redundancy	1.8	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6	20 *	TRIS-HCL, MAGNESIUM CHLORIDE, GDP, POLYETHYLENE GLYCOL 3350, AMMONIUM ACETATE, AMMONIUM CITRATE, PH 7.6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10.4 (mg/ml)
2	1	drop	PEG3350	20 (%(w/v))
3	1	drop	ammonium acetate	0.2 (M)
4	1	drop	ammonium citrate	0.1 (M)
5	1	reservoir	PEG8000	50 (%(w/v))