1D8E

Zinc-depleted FTase complexed with K-RAS4B peptide substrate and FPP analog.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	96
Detector technology	IMAGE PLATE
Collection date	1999-03-05
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 61
Unit cell lengths	171.411, 171.411, 69.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.199
Rwork	0.196
R-free	0.24900
RMSD bond length	0.010
RMSD bond angle	20.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.030
High resolution limit [Å]	3.000	3.000
Rmerge	0.090	0.289
Total number of observations	85082 *
Number of reflections	21199
<I/σ(I)>	7.9
Completeness [%]	89.3	82.2 *
Redundancy	4.01	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	17 *	Peg 8000, ammonium acetate, DTT , pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	12-16 (%(w/v))
2	1	reservoir	ammonium acetate	600 (mM)
3	1	reservoir	dithiothreitol	20 (mM)