1D5R
Crystal Structure of the PTEN Tumor Suppressor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 1998-12-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 4 |
| Unit cell lengths | 113.140, 113.140, 58.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.219 * |
| Rwork | 0.224 |
| R-free | 0.27600 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.037 | 0.163 |
| Total number of observations | 188143 * | |
| Number of reflections | 20302 | |
| <I/σ(I)> | 42.2 | |
| Completeness [%] | 95.8 | 76.4 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | NA/K TARTRATE, 5% GLYCEROL, 100MM TRIS-CL, 10MM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Na/K L(+)-tartrate | 1.3 (M) | |
| 2 | 1 | reservoir | glycerol | 5-10 (%(v/v)) | |
| 3 | 1 | reservoir | Tris-Cl | 100 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) |
