1CZ8
VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH AN AFFINITY MATURED ANTIBODY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-28 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.120, 66.400, 138.750 |
| Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.26700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 29.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 | 0.380 |
| Total number of observations | 209257 * | |
| Number of reflections | 61742 | 8900 * |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 97.4 | 96.7 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 293 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.8 (A280) | |
| 2 | 1 | drop | Tris-HCl | 30 (mM) | |
| 3 | 1 | drop | sodium chloride | 0.4 (M) | |
| 4 | 1 | reservoir | PEG | 15 (%) | |
| 5 | 1 | reservoir | isopropanol | 10 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | reservoir | MES | 0.2 (M) |
