1CXZ
CRYSTAL STRUCTURE OF HUMAN RHOA COMPLEXED WITH THE EFFECTOR DOMAIN OF THE PROTEIN KINASE PKN/PRK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-C |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-05 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.900, 66.900, 149.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.800 - 2.200 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.26800 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.450 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.800 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.312 |
Total number of observations | 696854 * | |
Number of reflections | 17568 | |
<I/σ(I)> | 24 | |
Completeness [%] | 96.0 | 86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.5 | 4 * | Maesaki, R., (1999) J.Struct.Biol., 126, 166. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 2 (mg/ml) | |
2 | 1 | 1 | Bis-Tris | 50 (mM) | |
3 | 1 | 1 | calcium acetate | 15 (mM) | |
4 | 1 | 1 | PEG300 | 15 (%) |