1CVR
Crystal structure of the Arg specific cysteine proteinase gingipain R (RGPB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.930, 79.920, 99.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
Rwork | 0.163 |
R-free | 0.20700 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.550 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.640 | 2.140 |
High resolution limit [Å] | 2.000 | 2.030 |
Rmerge | 0.078 | 0.231 |
Number of reflections | 25583 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.0 | 96.3 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15 % PEG 8000 100 MM ZINC ACETATE 100 MM SODIUM CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
2 | 1 | reservoir | PEG8000 | 15 (%) | |
3 | 1 | reservoir | zinc acetate | 0.1 (M) |