1CVR

Crystal structure of the Arg specific cysteine proteinase gingipain R (RGPB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1997-09-02
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	51.930, 79.920, 99.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
Rwork	0.163
R-free	0.20700
RMSD bond length	0.009
RMSD bond angle	1.550
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.640	2.140
High resolution limit [Å]	2.000	2.030
Rmerge	0.078	0.231
Number of reflections	25583
<I/σ(I)>	7.1
Completeness [%]	99.0	96.3
Redundancy	3.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	15 % PEG 8000 100 MM ZINC ACETATE 100 MM SODIUM CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium cacodylate	0.1 (M)
2	1	reservoir	PEG8000	15 (%)
3	1	reservoir	zinc acetate	0.1 (M)