1C8K
FLAVOPIRIDOL INHIBITS GLYCOGEN PHOSPHORYLASE BY BINDING AT THE INHIBITOR SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 287 |
| Collection date | 2000-03-01 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.400, 128.400, 116.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.500 - 1.760 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.053 | 0.640 |
| Total number of observations | 489494 * | |
| Number of reflections | 95442 | |
| <I/σ(I)> | 14.6 | 2 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.7 | pH 6.70 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 25 (mg/ml) | |
| 2 | 1 | 1 | flavopiridol | 1 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 4 | 1 | 1 | Bes | 10 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) | |
| 6 | 1 | 1 | sodium azide | 0.02 (%) | |
| 7 | 1 | 1 | spermine | 1 (mM) | or 10 mM magnesium acetate |
| 8 | 1 | 1 | glucose | 50 (mM) |
