1C3R
CRYSTAL STRUCTURE OF AN HDAC HOMOLOG COMPLEXED WITH TRICHOSTATIN A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.400, 94.400, 156.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.25800 |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.780 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.071 |
Total number of observations | 180427 * |
Number of reflections | 50796 * |
Completeness [%] | 93.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 * | microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | tert-butanol | 23 (%) | |
2 | 1 | reservoir | PEG1500 | 27 (%) | |
3 | 1 | reservoir | 600 (mM) | ||
4 | 1 | reservoir | bis-tris-propane-Cl | 100 (mM) |