1C2P
HEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-23 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.840, 105.200, 127.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.530 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 | 0.252 |
| Total number of observations | 404133 * | |
| Number of reflections | 85549 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 96.0 | 82 |
| Redundancy | 4.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5 | 295 | PEG 4000, GLYCEROL, DTT, MES, NACL, pH 5, BATCH, temperature 22K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 2 | 1 | 1 | 300 (mM) | ||
| 3 | 1 | 1 | glycerol | 10 (%(v/v)) | |
| 4 | 1 | 1 | dithiothreitol | 5 (mM) | |
| 5 | 1 | 1 | Tris | 5 (mM) | pH7.5 |
| 6 | 1 | 1 | PEG4000 | 10 (%(w/v)) | |
| 7 | 1 | 1 | MES | 25 (mM) | pH5.0 |
