1BXS
SHEEP LIVER CLASS 1 ALDEHYDE DEHYDROGENASE WITH NAD BOUND
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 80.689, 87.625, 90.840 |
Unit cell angles | 110.47, 105.63, 103.56 |
Refinement procedure
Resolution | 100.000 - 2.350 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ag8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.610 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.3C) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.096 | 0.350 |
Number of reflections | 83641 | |
<I/σ(I)> | 11.3 | 2.7 |
Completeness [%] | 95.5 | 93.7 |
Redundancy | 2.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 | pH 7.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | NAD+ | 1 (mM) | |
3 | 1 | reservoir | 170 (mM) | ||
4 | 1 | reservoir | Bis-Tris propane | 100 (mM) | |
5 | 1 | reservoir | mPEG5000 | 6-7 (%(w/v)) |