1BWN
PH DOMAIN AND BTK MOTIF FROM BRUTON'S TYROSINE KINASE MUTANT E41K IN COMPLEX WITH INS(1,3,4,5)P4
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-02 |
Detector | MARRESEARCH |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 110.784, 110.784, 215.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.100 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bwn |
RMSD bond length | 0.020 |
RMSD bond angle | 19.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.037 * | 0.322 * |
Number of reflections | 39294 | |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 3.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | pH 5.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
2 | 1 | reservoir | Na acetate | 100 (mM) | |
3 | 1 | drop | protein | 10 (mg/ml) |