1BQQ
CRYSTAL STRUCTURE OF THE MT1-MMP--TIMP-2 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.650, 40.100, 85.680 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.750 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.880 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.095 | 0.389 |
| Total number of observations | 73215 * | |
| Number of reflections | 11911 | |
| Completeness [%] | 97.0 | 93.2 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | ammonium sulfate | 100 (mM) | |
| 7 | 1 | drop | PEG8000 | 16 (%(w/v)) | |
| 8 | 1 | drop | MES-NaOH | 5.6 |
