1BJG
D221(169)N MUTANT DOES NOT PROMOTE OPENING OF THE COFACTOR IMIDAZOLIDINE RING
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 294 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01 |
Detector | RIGAKU |
Spacegroup name | I 21 3 |
Unit cell lengths | 133.000, 133.000, 133.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.300 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WILD TYPE E. COLI TS |
RMSD bond length | 0.017 |
RMSD bond angle | 24.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.370 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.250 |
Total number of observations | 68196 * | |
Number of reflections | 16757 | |
<I/σ(I)> | 10.4 | 3.6 |
Completeness [%] | 94.9 | 90.1 |
Redundancy | 4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.7 | pH 7.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 20 (mM) | |
3 | 1 | drop | ammonium sulfate | 2.3 (M) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | FdUMP | 20 (mM) | |
6 | 1 | drop | CH2H4folate | 10 (mM) |