1BI7
MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURE OF THE CDK6-P16INK4A TUMOR SUPPRESSOR COMPLEX
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1997-01 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 123.100, 123.100, 112.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.400 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.33000 |
Structure solution method | MIR, MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.940 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.068 * | 0.330 * |
Total number of observations | 68633 * | |
Number of reflections | 12443 | |
<I/σ(I)> | 17.7 | 6.9 |
Completeness [%] | 97.7 | 96.2 |
Redundancy | 5.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | isopropanol | 11 (%) | |
2 | 1 | reservoir | HEPES-Na | 40 (mM) | |
3 | 1 | reservoir | dithiothreitol | 5 (mM) |