1AFC

STRUCTURAL STUDIES OF THE BINDING OF THE ANTI-ULCER DRUG SUCROSE OCTASULFATE TO ACIDIC FIBROBLAST GROWTH FACTOR

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	110.600, 110.600, 172.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.700
R-factor	0.204
Rwork	0.204
RMSD bond length	0.016
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.118 *
Total number of observations	138212 *
Number of reflections	31326 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	10 (mg/ml)
2	1	reservoir		0.1 (M)
3	1	reservoir	PEG8000	27.5 (%)
4	1	reservoir	N-2-acetamidoiminodiacetic	0.1 (M)
5	1	reservoir	SOS	3.3 (mM)