1A9X
CARBAMOYL PHOSPHATE SYNTHETASE: CAUGHT IN THE ACT OF GLUTAMINE HYDROLYSIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1997-11 |
| Detector | BRUKER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 152.100, 164.400, 332.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jdb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 17.700 * |
| Data reduction software | SAINT (2000) |
| Data scaling software | XCALIBRE |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.275 |
| Total number of observations | 1676492 * | |
| Number of reflections | 700998 | 85888 * |
| <I/σ(I)> | 23.8 | 4 |
| Completeness [%] | 92.0 | 91 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 * | 277 * | Thoden, J.B., (1995) Acta Crysta., D51, 827. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 100 (mM) | ||
| 2 | 1 | 1 | L-ornithine | 0.5 (mM) | |
| 3 | 1 | 1 | 0.5 (mM) | ||
| 4 | 1 | 1 | ADP | 2.5 (mM) | |
| 5 | 1 | 1 | PEG8000 | 8 (%) | |
| 6 | 1 | 1 | tetraethylammonium chloride | 0.65 (M) | |
| 7 | 1 | 1 | HEPPS | 12.5 (mM) | |
| 8 | 1 | 1 | 2.5 (mM) | ||
| 9 | 1 | 1 | glutamine | 5 (mM) |
