1A5B
CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHA D60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.600, 59.700, 67.400 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28300 |
| Structure solution method | ISOMORPHOUS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.050 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 * | 0.424 * |
| Number of reflections | 45501 | |
| <I/σ(I)> | 14.7 | 2.4 |
| Completeness [%] | 92.8 | 79.4 * |
| Redundancy | 4.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.8 | 50MM NABICINE (PH 7.8), 1MM NA-EDTA, 0.8-1.5MM SPERMINE, 12% PEG8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | N,N-bis(2-hydroxyethyl)glycine | 50 (mM) | |
| 2 | 1 | 1 | Na-EDTA | 1 (mM) | |
| 3 | 1 | 1 | spermine | 0.8-1.5 (mM) | |
| 4 | 1 | 1 | PEG8000 | 12 (%) |
