1A4I

HUMAN TETRAHYDROFOLATE DEHYDROGENASE / CYCLOHYDROLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1996-10
Detector	PRINCETON 2K
Spacegroup name	P 21 21 21
Unit cell lengths	67.523, 135.843, 61.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.199 *
Rwork	0.199
R-free	0.23500
Structure solution method	MAD SELENOMET
RMSD bond length	0.015
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall
High resolution limit [Å]	1.500
Rmerge	0.068
Total number of observations	753128 *
Number of reflections	75578
Completeness [%]	90.0
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	18 *	pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	sodium citrate	0.1 (mM)
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	reservoir	PEG3350	21 (%)
5	1	reservoir	glycerol	5 (%)