1A02
STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-16 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.660, 85.460, 83.370 |
Unit cell angles | 90.00, 112.03, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.700 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.30300 |
Structure solution method | MIR/MAD |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.620 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.080 * | 0.430 * |
Number of reflections | 22079 | |
Completeness [%] | 98.3 | 93.3 |
Redundancy | 3.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | THE COMPLEX WAS CRYSTALLIZED IN 300-400 MM AMMONIUM ACETATE SALT, PH 7.5 (10 MM)., VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | HEPES | ||
10 | 1 | 2 | AMMINO ACETATE | ||
2 | 1 | 1 | DTT | ||
3 | 1 | 1 | NACL | ||
4 | 1 | 1 | GLYCEROL | ||
5 | 1 | 1 | AMMINO ACETATE | ||
6 | 1 | 2 | HEPES | ||
7 | 1 | 2 | DTT | ||
8 | 1 | 2 | NACL | ||
9 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 0.2-0.4 (mM) | |
2 | 1 | reservoir | HEPES | 10 (mM) | |
3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
4 | 1 | reservoir | 100 (mM) | ||
5 | 1 | reservoir | glycerol | 20 (%) | |
6 | 1 | reservoir | 300 (mM) |