1ZXM
Human Topo IIa ATPase/AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2004-05-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.772, 75.285, 164.887 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.870 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.078 | |
| Number of reflections | 70576 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 98.5 | 96.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 277 | PEG 6000, Tris chloride, magnesium chloride, DTT, BME, AMP-PNP., pH 7.4, VAPOR DIFFUSION, temperature 277K |
