1Z5N
Crystal structure of MTA/AdoHcy nucleosidase Glu12Gln mutant complexed with 5-methylthioribose and adenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.680, 69.460, 128.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.780 - 2.100 |
| R-factor | 0.2051 |
| Rwork | 0.205 |
| R-free | 0.25200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nc1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.780 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.084 |
| Number of reflections | 51948 |
| Completeness [%] | 99.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | PEG 4000, sodium acetate, glycerol, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
