1YVJ

Crystal structure of the Jak3 kinase domain in complex with a staurosporine analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2004-12-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.307, 54.215, 118.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.970 - 2.550
R-factor	0.2041
Rwork	0.204
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m17
RMSD bond length	0.011
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.970	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	9949
Completeness [%]	97.9	97.4
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	malonate, glycerol, pipes, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K