1YTU
Structural basis for 5'-end-specific recognition of the guide RNA strand by the A. fulgidus PIWI protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9776 |
Spacegroup name | H 3 |
Unit cell lengths | 238.747, 238.747, 52.042 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.20689 |
Rwork | 0.204 |
R-free | 0.26623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w9h |
RMSD bond length | 0.017 |
RMSD bond angle | 1.792 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.046 | 0.279 |
Number of reflections | 38254 | |
Completeness [%] | 99.3 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 4K, potassium chloride, magnisium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4K | ||
2 | 1 | 1 | potassium chloride | ||
3 | 1 | 1 | magnisium chloride | ||
4 | 1 | 1 | cacodylate | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | potassium chloride | ||
7 | 1 | 2 | magnisium chloride | ||
8 | 1 | 2 | PEG 4K |