1YKB

Crystal Structure of Insect Cell Expressed IL-22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.255
Spacegroup name	P 1 21 1
Unit cell lengths	64.877, 62.227, 139.524
Unit cell angles	90.00, 91.35, 90.00

Refinement procedure

Resolution	19.930 - 2.600
Rwork	0.232
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	BEAST
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.950
High resolution limit [Å]	2.500
Number of reflections	33239

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	310	pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K