1YGU
Crystal structure of the tandem phosphatase domains of RPTP CD45 with a pTyr peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-03 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.900, 57.920, 159.290 |
| Unit cell angles | 90.00, 98.66, 90.00 |
Refinement procedure
| Resolution | 29.160 - 2.900 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D2 domain of RPTP LAR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.273 | |
| Number of reflections | 32025 | |
| <I/σ(I)> | 17.9 | 2.1 |
| Completeness [%] | 92.8 | 67 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 4000, sodium acetate, glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
