1YGR

Crystal structure of the tandem phosphatase domain of RPTP CD45

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	200
Detector technology	DIFFRACTOMETER
Collection date	2002-12-15
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	86.010, 59.700, 160.530
Unit cell angles	90.00, 99.53, 90.00

Refinement procedure

Resolution	29.850 - 2.900
R-factor	0.255
Rwork	0.255
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.850
High resolution limit [Å]	2.900
Number of reflections	36165

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	Sodium acetate, PEG 4000, Glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K