1YFJ
T4Dam in Complex with AdoHcy and 15-mer Oligonucleotide Showing Semi-specific and Specific Contact
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 173 |
Detector technology | AREA DETECTOR |
Collection date | 2003-12-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.200, 133.000, 189.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.780 - 2.690 |
R-factor | 0.206 |
Rwork | 0.202 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q0s |
RMSD bond length | 0.010 |
RMSD bond angle | 2.000 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 78518 | |
<I/σ(I)> | 12.9 | 7.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.3 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | PEG 6000, MES, ammonium acetate, CaCl2, and ethyleneglycol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | ammonium acetate | ||
4 | 1 | 1 | CaCl2 | ||
5 | 1 | 1 | ethyleneglycol | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 6000 | ||
8 | 1 | 2 | ammonium acetate | ||
9 | 1 | 2 | CaCl2 |