1YC3
Crystal Structure of human HSP90alpha complexed with dihydroxyphenylpyrazoles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-19 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.775, 44.829, 53.157 |
| Unit cell angles | 90.00, 114.62, 90.00 |
Refinement procedure
| Resolution | 48.330 - 2.120 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.330 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.120 |
| Rmerge | 0.114 | 0.320 |
| Number of reflections | 12896 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 99.2 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | PEG 6000, LiCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 9.00 |
