1YA3
Crystal structure of the human mineralocorticoid receptor ligand-binding domain bound to progesterone and harboring the S810L mutation responsible for a severe form of hypertension
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2004-11-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97950 |
Spacegroup name | P 31 |
Unit cell lengths | 120.724, 120.724, 42.508 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.720 - 2.340 |
R-factor | 0.252 |
Rwork | 0.237 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y9r |
RMSD bond length | 0.015 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BEAST |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.730 | 2.470 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.076 | 0.311 |
Number of reflections | 22222 | |
<I/σ(I)> | 8.6 | 2.8 |
Completeness [%] | 81.7 | 80.3 |
Redundancy | 2.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | PEG 4000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |