1Y97

The human TREX2 3' exonuclease structure suggests a mechanism for efficient non-processive DNA catalysis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-30
Detector	BRANDEIS - B4
Wavelength(s)	0.97832, 0.97876, 0.9500
Spacegroup name	P 21 21 21
Unit cell lengths	52.500, 77.200, 101.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.205
Rwork	0.205
R-free	0.26100
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.550
High resolution limit [Å]	2.470	2.470
Rmerge	0.078	0.180
Number of reflections	14344
<I/σ(I)>	19	4
Completeness [%]	97.0	80
Redundancy	5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	303	0.4 M ammonium phosphate, 7% PEG400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 303K