1W7K

E.coli FolC in complex with ADP, without folate substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-09-19
Detector	MACSCIENCE DIP2000
Spacegroup name	P 21 21 21
Unit cell lengths	66.112, 81.602, 93.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.160 - 2.100
Rwork	0.177
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w78
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.200	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.110	0.290
Number of reflections	29894
<I/σ(I)>	5.8	2.5
Completeness [%]	99.1	100
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.7		1.5 M AMMONIUM SULFATE 5 MM DTT 50 MM BICINE PH 8.7