1W72
Crystal structure of HLA-A1:MAGE-A1 in complex with Fab-Hyb3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 125.359, 50.050, 136.705 |
| Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
| Resolution | 120.000 - 2.150 |
| R-factor | 0.186 |
| Rwork | 0.182 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1DUZ 7fab 2gfb 2fb4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.475 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 78453 | |
| <I/σ(I)> | 15 | 2.3 |
| Completeness [%] | 90.0 | 90 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MES PH 6.5, NACL 600MM, 19% PEG3350, 80MM DIOXANE |
