1VG9
The crystal structures of the REP-1 protein in complex with C-terminally truncated Rab7 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91847 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.392, 144.691, 200.086 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 19.420 - 2.500 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vg0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 129867 | |
| <I/σ(I)> | 17.99 | 6.06 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 23% PEG 2000 MME, 0.4M Potassium Formate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
