1UPU

STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE, MUTANT C128V, BOUND TO PRODUCT URIDINE-1-MONOPHOSPHATE (UMP)

Experimental procedure

Temperature [K]	293
Collection date	1997-08
Spacegroup name	P 1 21 1
Unit cell lengths	60.280, 141.770, 71.420
Unit cell angles	90.00, 114.93, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.146 *
Rwork	0.148
RMSD bond length	0.019 *
RMSD bond angle	1.990 *
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.033 *
Total number of observations	53734 *
Number of reflections	32670 *
Completeness [%]	81.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop	dithiothreitol	10 (mM)
3	1	drop	protein	25-40 (mg/ml)
4	1	reservoir	ammonium phosphate	1.0 (M)
5	1	reservoir	citrate	0.1 (M)
6	1	reservoir		0.2 (M)