1U32

Crystal structure of a Protein Phosphatase-1: Calcineurin Hybrid Bound to Okadaic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-07-22
Detector	RIGAKU RAXIS IV
Spacegroup name	P 42 21 2
Unit cell lengths	98.759, 98.759, 62.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1JK7
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.076	0.369
Number of reflections	21304
<I/σ(I)>	21.1	5.02
Completeness [%]	97.3	99
Redundancy	5.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	lithium sulfate, tris, PEG 400, mercaptoethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K