1U2Z
Crystal structure of histone K79 methyltransferase Dot1p from yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.070, 146.275, 75.412 |
| Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.200 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.302 | |
| Number of reflections | 79720 | |
| <I/σ(I)> | 23.2 | 4.1 |
| Completeness [%] | 98.7 | 94.1 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | PEG 8000, Glycerol, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
