1TZG
Crystal structure of HIV-1 neutralizing human Fab 4E10 in complex with a 13-residue peptide containing the 4E10 epitope on gp41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-02-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979126 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.300, 45.100, 198.500 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 43.000 - 2.200 |
| Rwork | 0.217 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hkl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 61572 | |
| <I/σ(I)> | 16.7 | 2.3 |
| Completeness [%] | 93.0 | 61.4 |
| Redundancy | 3.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | PEG 8000, sodium acetate, hexamine cobalt trichloride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
