1TC2
TERNARY SUBSTRATE COMPLEX OF THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE FROM TRYPANOSOMA CRUZI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.420, 101.760, 51.920 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.810 |
| R-factor | 0.191 |
| Rwork | 0.183 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.480 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.600 | 1.850 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.051 * | 0.320 * |
| Number of reflections | 33026 | |
| <I/σ(I)> | 20.8 | 3.8 |
| Completeness [%] | 88.8 | 80.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PRPP | 5 (mM) | |
| 3 | 1 | drop | HPP | 5 (mM) | |
| 4 | 1 | drop | bis-tris | 20 (mM) | |
| 5 | 1 | drop | 6 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | PEG4000 | 30 (%) |
