1T08

Crystal structure of beta-catenin/ICAT helical domain/unphosphorylated APC R3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	96.472, 96.712, 86.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 2.100
R-factor	0.215
Rwork	0.204
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m1e
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.296
Number of reflections	47853
<I/σ(I)>	15.2	2.16
Completeness [%]	95.2	90.5
Redundancy	2.67	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	297	PEG3400, Potassium phosphate, pH 6.5, EVAPORATION, temperature 297K
1	EVAPORATION	6.5	297	PEG3400, Potassium phosphate, pH 6.5, EVAPORATION, temperature 297K
1	EVAPORATION	6.5	297	PEG3400, Potassium phosphate, pH 6.5, EVAPORATION, temperature 297K