1T02

Crystal structure of a Statin bound to class II HMG-CoA reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	I 41 3 2
Unit cell lengths	228.500, 228.500, 228.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.600
Rwork	0.196
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qax
RMSD bond length	0.016
RMSD bond angle	1.850
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.600
Number of reflections	26296
<I/σ(I)>	15
Completeness [%]	87.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K