1SXJ

Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9464, 0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	104.209, 110.481, 268.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.810 - 2.850
R-factor	0.251
Rwork	0.251
R-free	0.30600
Structure solution method	MAD
Starting model (for MR)	1plq 1jr3 1njf 1iqp
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	132895
<I/σ(I)>	22.7	2.7
Completeness [%]	100.0	100
Redundancy	9.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	20 *	PEG 3350, sodium chloride, CHES, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	9	20 *	PEG 3350, sodium chloride, CHES, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30-50 (mg/ml)
2	1	reservoir		300 (mM)
3	1	reservoir	PEG3350	14.4-15.2 (%(w/v))
4	1	reservoir	CHES	100 (mM)
5	1	reservoir	beta-mercaptoethanol	5 (mM)
6	1	drop	ATP-gammaS	10 (mM)
7	1	drop		10 (mM)
8	1	drop	dithiothreitol	4 (mM)
9	1	drop	TCEP	2 (mM)