1STX
Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.710, 58.690, 82.100 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.2012 |
| Rwork | 0.201 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.010 |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 32988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290 | 17% PEG 4000, 0.1M HEPES pH 7.5, 0.15M NaCl 50mM MnCl2, Vapor diffusion, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | MnCl2 |
