1SQ0

Crystal Structure of the Complex of the Wild-type Von Willebrand Factor A1 domain and Glycoprotein Ib alpha at 2.6 Angstrom Resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97942, 0.97959, 0.9686
Spacegroup name	P 21 21 2
Unit cell lengths	72.497, 116.104, 67.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 2.600
R-factor	0.19
Rwork	0.190
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	2.750
High resolution limit [Å]	2.600	2.600
Number of reflections	17150
Completeness [%]		84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8	291	PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K