1SKM

HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	H 3 2
Unit cell lengths	95.681, 95.681, 315.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.530 - 2.200
R-factor	0.183
Rwork	0.183
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	CAD4
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.530	2.250
High resolution limit [Å]	2.200	2.200
Rmerge		0.179
Number of reflections	23477
<I/σ(I)>	18.4
Completeness [%]	81.9	36.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	PEG 6000, sodium cacodylate, magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 6000
2	1	1	sodium cacodylate
3	1	1	magnesium acetate