1SI2

Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex of deoxynucleotide overhang

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-13
Detector	MARRESEARCH
Wavelength(s)	0.9810
Spacegroup name	P 64
Unit cell lengths	100.051, 100.051, 34.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.710 - 2.600
R-factor	0.2247
Rwork	0.221
R-free	0.26100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	6225
<I/σ(I)>	27.8	3.1
Completeness [%]	99.0	100
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	PEG 1000, potassium chloride, HEPES-NaOH, Dithiothreitol, pH 7.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 1000
2	1	1	potassium chloride
3	1	1	HEPES-NaOH
4	1	1	Dithiothreitol