1RE0

Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.725, 72.294, 119.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.400
R-factor	0.209
Rwork	0.209
R-free	0.23800 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.061	0.249 *
Total number of observations	136824 *
Number of reflections	22531	2221 *
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	PEG 400, sodium citrate, Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	50 (mM)
2	1	reservoir	PEG400	19 (%(w/v))
3	1	reservoir	Tris-HCl	100 (mM)	pH8.5
4	1	drop	HEPES	20 (mM)
5	1	drop		150 (mM)
6	1	drop	dithiothreitol	4 (mM)