1R8Q
FULL-LENGTH ARF1-GDP-MG IN COMPLEX WITH BREFELDIN A AND A SEC7 DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.081, 109.773, 118.326 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.860 |
| R-factor | 0.18111 |
| Rwork | 0.179 |
| R-free | 0.22200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.971 |
| Data scaling software | XDS |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.880 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 74905 | |
| <I/σ(I)> | 18.6 | 3.1 |
| Completeness [%] | 83.6 | |
| Redundancy | 11.77 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 16 * | 19% Peg 3350, 175mM NH4 Fluoride, 100mM cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 19 (%) | |
| 2 | 1 | reservoir | ammonium fluoride | 175 (mM) | |
| 3 | 1 | reservoir | cacodylate | 100 (mM) | pH6.5 |
| 4 | 1 | reservoir | sucrose | 17 (%) |
